Thermo Fisher Scientific CD11c Monoclonal Antibody (3.9), PE-Cyanine7, eBioscience

Thermo Fisher Scientific CD11c Monoclonal Antibody (3.9), PE-Cyanine7, eBioscience™

Applications

• Flow Cytometry (Flow)
  Tested Dilution: 5 µL (1 µg)/test
  View 29 publications

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 532
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 737 / 805
• eFluor 450 / 506
• FITC
• PE
• PE-Cyanine5 / 5.5
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 600 / 645 / 702 / 780
• Request custom conjugation available

Product Specific Information

Description:
The 3.9 monoclonal antibody reacts with human CD11c, the 150 kDa integrin alpha X chain. CD11c non-covalently associates with beta2 integrin to form the CD11c/CD18 heterodimer. This complex is expressed on monocytes, granulocytes, macrophages, NK, dendritic cells, and subsets of T and B lymphocytes. CD11c/CD18 binds to CD54, iC3b, and fibrinogen and plays a role in leukocyte adhesive interactions.

Applications Reported:
This 3.9 antibody has been reported for use in flow cytometric analysis.

Applications Tested:
Pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. Use at 5 µL (1 µg) per test (100 µL final volume). Suitable for 10⁵–10⁸ cells/test.

Light Sensitivity:
This tandem dye is sensitive to photo-induced oxidation. Protect vial and stained samples from light.

Fixation:
Samples can be stored in IC Fixation Buffer (Product #00-822-49) or 1-step Fix/Lyse Solution (Product #00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency.

Excitation/Emission:
488–561 nm excitation; 775 nm emission. Compatible with Blue, Green, and Yellow-Green lasers.

Filtration:
0.2 µm post-manufacturing filtered.

Target Information

CD11, along with CD18, form a heterodimer adhesion molecule.

• CD11a: Expressed in B and T lymphocytes, macrophages, monocytes, neutrophils, basophils, eosinophils.
• CD11b: Expressed by monocytes, macrophages, NK cells, some B/T cells, and granulocytes.
• CD11c: Expressed in monocytes, macrophages, NK cells, granulocytes, and subsets of lymphocytes.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

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