Thermo Fisher Scientific Arginase 1 Monoclonal Antibody (sl6arg), Alexa Fluor 488, eBioscience

Thermo Fisher Scientific Arginase 1 Monoclonal Antibody (sl6arg), Alexa Fluor 488, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 514
• Alexa Fluor 700
• Alexa Fluor Plus 488
• Alexa Fluor Plus 555
• eFluor 660
• Request custom conjugation available

Product Specific Information

The monoclonal antibody sl6arg recognizes human Arginase-1, a 35 kDa enzyme that eliminates nitrogen by converting L-arginine to urea and L-ornithine, which further converts to polyamines. These polyamines are crucial for cell proliferation and detoxification from protein degradation. Expression is found in the liver, neutrophils, myeloid-derived suppressor cells (MDSC), and neural stem cells. In humans, expression in macrophages has not been determined, while in mice, M2 macrophages express Arginase-1 strongly. It may also be expressed in myeloid populations within breast cancer tumors and is typically found in hepatocellular carcinomas.

Applications Reported: Suitable for immunohistochemical staining of formalin-fixed paraffin-embedded tissue sections, microscopy, and immunocytochemistry.
Applications Tested: Validated for use in immunocytochemistry of formaldehyde-fixed and permeabilized cells at ≤20 µg/mL. Optimal titration recommended for specific assays.
Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Arginase-1 (Arg1) is a 35 kDa enzyme converting L-arginine to urea and L-ornithine, the final step in the urea cycle. Resulting polyamines are important for cell proliferation and detoxification. By degrading arginine, Arginase 1 deprives NO synthase of its substrate, down-regulating nitric oxide production. Expressed in liver, neutrophils, MDSCs, and neural stem cells. In humans, expression occurs in blood neutrophils but not in CCR3+ granulocytes. In mice, Arginase 1 is a hallmark of alternatively activated macrophages (M2a). Expression may occur in tumor-infiltrating myeloid cells and is typically found in hepatocellular carcinomas. Defects in Arginase 1 cause argininemia, an autosomal recessive disorder characterized by hyperammonemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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