Thermo Fisher Scientific Arginase 1 Monoclonal Antibody (sl6arg), Alexa Fluor 488, eBioscience
인간 Arginase-1 단백질을 인식하는 단클론 항체로, Alexa Fluor 488 형광으로 표지되어 있습니다. 세포 및 조직 면역염색(IHC, ICC/IF)에 적합하며, 488/519 nm의 청색 레이저에서 최적의 형광 신호를 제공합니다. 간, 신경줄기세포, MDSC 등에서의 Arginase-1 발현 연구에 활용됩니다.
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Thermo Fisher Scientific Arginase 1 Monoclonal Antibody (sl6arg), Alexa Fluor 488, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.25 µg/mL |
| Immunohistochemistry (IHC) | - |
| Immunohistochemistry (Paraffin) (IHC (P)) | 5–20 µg/mL |
| Immunocytochemistry (ICC/IF) | 20 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Alexa Fluor™ 488, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | sl6arg |
| Conjugate | Alexa Fluor™ 488 |
| Excitation / Emission Max | 499 / 520 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2574476 |
Additional Formats
- Unconjugated
- Alexa Fluor 514
- Alexa Fluor 700
- Alexa Fluor Plus 488
- Alexa Fluor Plus 555
- eFluor 660
- Request custom conjugation available
Product Specific Information
The monoclonal antibody sl6arg recognizes human Arginase-1, a 35 kDa enzyme that eliminates nitrogen by converting L-arginine to urea and L-ornithine, which further converts to polyamines. These polyamines are crucial for cell proliferation and detoxification from protein degradation. Expression is found in the liver, neutrophils, myeloid-derived suppressor cells (MDSC), and neural stem cells. In humans, expression in macrophages has not been determined, while in mice, M2 macrophages express Arginase-1 strongly. It may also be expressed in myeloid populations within breast cancer tumors and is typically found in hepatocellular carcinomas.
Applications Reported: Suitable for immunohistochemical staining of formalin-fixed paraffin-embedded tissue sections, microscopy, and immunocytochemistry.
Applications Tested: Validated for use in immunocytochemistry of formaldehyde-fixed and permeabilized cells at ≤20 µg/mL. Optimal titration recommended for specific assays.
Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Arginase-1 (Arg1) is a 35 kDa enzyme converting L-arginine to urea and L-ornithine, the final step in the urea cycle. Resulting polyamines are important for cell proliferation and detoxification. By degrading arginine, Arginase 1 deprives NO synthase of its substrate, down-regulating nitric oxide production. Expressed in liver, neutrophils, MDSCs, and neural stem cells. In humans, expression occurs in blood neutrophils but not in CCR3+ granulocytes. In mice, Arginase 1 is a hallmark of alternatively activated macrophages (M2a). Expression may occur in tumor-infiltrating myeloid cells and is typically found in hepatocellular carcinomas. Defects in Arginase 1 cause argininemia, an autosomal recessive disorder characterized by hyperammonemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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