Thermo Fisher Scientific ATP5J Polyclonal Antibody
ATP5J 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal 항체. Western blot, IHC, ICC에 적합하며 Human, Mouse, Rat에 반응. 항원 친화 크로마토그래피로 정제된 액상 제품으로, 4°C 단기 보관 및 -20°C 장기 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Full length human ATP5J recombinant protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.01% thimerosal |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_2546678 |
Product Specific Information
- Recommended positive controls: HeLa, HeLa membrane
- Predicted reactivity: Mouse (80%), Rhesus Monkey (91%), Bovine (80%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It consists of two linked multi-subunit complexes: the soluble catalytic core (F1) and the membrane-spanning component (F0).
The F1 complex includes five subunits (α, β, γ, δ, ε) in a ratio of 3α:3β:1γ:1δ:1ε.
The F0 complex contains nine subunits (a, b, c, d, e, f, g, F6, and 8).
The ATP5J gene encodes the F6 subunit of the F0 complex, required for F1–F0 interactions.
Alternatively spliced transcript variants encoding different isoforms have been identified.
Safety Information
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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