Thermo Fisher Scientific PRDX4 Recombinant Rabbit Monoclonal Antibody (SAIC-40A-24)
PRDX4 단백질을 인식하는 재조합 토끼 단클론 항체로, Western blot, ICC/IF, Flow cytometry, ELISA, Mass spectrometry에 사용 가능. HEK293 세포에서 발현되었으며 Protein A로 정제됨. 인간 PRDX4에 특이적이며 연구용으로 적합.
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Thermo Fisher Scientific PRDX4 Recombinant Rabbit Monoclonal Antibody (SAIC-40A-24)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.001 µg/mL |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
| ELISA | Assay-dependent |
| Mass Spectrometry (MS) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SAIC-40A-24 |
| Immunogen | Peptide QITLNDLPVGR, derived from the cell redox-regulator Peroxiredoxin-4, conjugated to KLH |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_3074363 |
Product Specific Information
Specificity: Recognises human PRDX4.
Target Information
Peroxiredoxin (Prx) is a growing peroxidase family, whose mammalian members are associated with cell proliferation, differentiation, and apoptosis. Many isoforms (about 50 proteins) share amino acid sequence homology and contain active site cysteine residues with thiol-specific antioxidant activity, distributed throughout all kingdoms.
Mammalian Prx consists of six members grouped into typical 2-Cys, atypical 2-Cys, and 1-Cys Prx. Except Prx VI (1-Cys subgroup), the other five 2-Cys Prx isotypes have thioredoxin-dependent peroxidase (TPx) activity utilizing thioredoxin, thioredoxin reductase, and NADPH as a reducing system. Mammalian Prxs (20–30 kDa) vary in subcellular localization:
- Prx I, II, and VI in cytosol
- Prx III in mitochondria
- Prx IV in ER and secretion
- Prx V in peroxisome, mitochondria, and cytosol
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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