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Thermo Fisher Scientific Phospho-ATM (Ser1981) Monoclonal Antibody (10H11.E12), eBioscience

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ATM 단백질의 Ser1981 인산화 형태를 특이적으로 인식하는 단클론 항체. Western blot, IHC, ICC, Flow cytometry, IP 등 다양한 응용 가능. DNA 손상 반응 연구 및 세포주기 조절 기전 분석에 유용. Human, Mouse, Rat 반응성. 4°C 보관.

브랜드: Thermo Fisher Scientific
카테고리: Monoclonal Antibodies
판매단위: pk

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장비

Thermo Fisher Scientific 14-9046-37 Phospho-ATM (Ser1981) Monoclonal Antibody (10H11.E12), eBioscience 2 mg pk판매 단위 pk ·

재고 확인 필요재고 확인 필요

2,990,200원VAT 포함 3,289,220원

소모품

Thermo Fisher Scientific 14-9046-82 Phospho-ATM (Ser1981) Monoclonal Antibody (10H11.E12), eBioscience 100 ug pk판매 단위 pk ·

재고 확인 필요재고 확인 필요

460,500원VAT 포함 506,550원

Thermo Fisher Scientific 14-9046-80 Phospho-ATM (Ser1981) Monoclonal Antibody (10H11.E12), eBioscience 25 ug pk판매 단위 pk ·

재고 확인 필요재고 확인 필요

186,800원VAT 포함 205,480원

재고문의·견적요청 옵션은 실시간 상담으로 납기를 확인해 드립니다 · 연구비 카드 결제 가능

Thermo Fisher Scientific · Thermo Fisher Scientific Phospho-ATM (Ser1981) Monoclonal Antibody (10H11.E12), eBioscience

Applications and Tested Dilutions

Application	Tested Dilution
Western Blot (WB)	Assay-dependent
Immunohistochemistry (Paraffin) (IHC-P)	10 µg/mL
Immunocytochemistry (ICC/IF)	10 µg/mL
Flow Cytometry (Flow)	Assay-dependent
Immunoprecipitation (IP)	Assay-dependent

Product Specifications

항목	내용
Species Reactivity	Human, Mouse, Rat
Published Species	Human
Host / Isotype	Mouse / IgG1, kappa
Class	Monoclonal
Type	Antibody
Clone	10H11.E12
Conjugate	Unconjugated
Form	Liquid
Concentration	0.5 mg/mL
Purification	Affinity chromatography
Storage Buffer	PBS, pH 7.2
Contains	0.09% sodium azide
Storage Conditions	4°C
Shipping Conditions	Ambient (domestic); Wet ice (international)
RRID	AB_2865325

Product Specific Information

This 10H11.E12 monoclonal antibody recognizes human, mouse, and rat ataxia-telangiectasia mutated (ATM) protein phosphorylated at serine 1981. ATM is a PI3 kinase family member that coordinates cell cycle arrest and DNA repair initiation through phosphorylation of multiple substrates in response to DNA damage and oxidative stress.

ATM exists as inactive dimers or multimers but becomes activated by monomer formation and autophosphorylation upon DNA double-strand breaks. Activated ATM halts the cell cycle and initiates DNA repair. Loss of ATM function leads to ataxia-telangiectasia (AT), characterized by neurodegeneration, immunodeficiency, radiosensitivity, and cancer predisposition (e.g., lymphoma, leukemia).

Applications Reported:
Suitable for intracellular staining followed by flow cytometry, immunoprecipitation, western blotting, immunocytochemistry, and immunohistochemistry of formalin-fixed paraffin-embedded tissues. Fluorochrome-conjugated 10H11.E12 is recommended for intracellular flow cytometry.

Applications Tested:

IHC (paraffin): ≤10 µg/mL (high or low pH antigen retrieval)
ICC: ≤10 µg/mL (methanol or formaldehyde-fixed cells)
Careful titration is recommended for optimal assay performance.

Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered

Target Information

Ataxia-telangiectasia Mutated (ATM) is a PI3/PI4 kinase family protein. The ATM gene encodes a kinase that phosphorylates numerous downstream targets, including P53, Mdm2, BRCA1, and SMC1, regulating cell cycle and DNA repair. ATM is activated by autophosphorylation following DNA damage such as ionizing radiation. Deficiency in ATM results in hypersensitivity to radiation, impaired DNA synthesis inhibition, delayed p53 induction, and dysregulated apoptosis via the E2F1 pathway involving Nbs1 and Chk2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.