Thermo Fisher Scientific VHL/HRCA1 Polyclonal Antibody
VHL/HRCA1 단백질을 인식하는 토끼 폴리클로날 항체로, 인간·마우스·랫에서 반응합니다. IHC, ICC/IF, Flow Cytometry, ELISA 등 다양한 응용에 적합합니다. Protein A로 정제된 액상 형태이며, 안정적인 TBS/BSA/glycerol buffer에 보관됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:400 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:20–1:100 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic peptide derived from human VHL, amino acids 101–213 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | –20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Von Hippel-Lindau syndrome (VHL) is a dominantly inherited familial cancer syndrome predisposing to various malignant and benign tumors. A germline mutation of this gene underlies the familial inheritance of VHL syndrome.
The protein encoded by this gene forms part of a complex including elongin B, elongin C, and cullin-2, possessing ubiquitin ligase E3 activity. It is involved in the ubiquitination and degradation of hypoxia-inducible factor (HIF), a transcription factor central to oxygen-regulated gene expression.
RNA polymerase II subunit POLR2G/RPB7 is also reported as a target. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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