Thermo Fisher Scientific GLI1 Monoclonal Antibody (OTI12D5), TrueMAB
인간 GLI1 단백질(aa 755-1106)에 특이적인 Mouse IgG1 단일클론 항체. Western blot에 최적화된 TrueMAB 형식. 무보존제, 동결건조 형태로 제공되며 재구성 후 1 mg/mL 농도 유지. 연구용으로만 사용 가능.
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Applications
- Western Blot (WB)
Tested Dilution
- 1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI12D5 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 755–1106 of human GLI1 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step.
Recommended product: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL (Product #89882).
Target Information
This gene encodes a member of the Kruppel family of zinc finger proteins. The encoded transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation.
The activity and nuclear localization of this protein are negatively regulated by p53 in an inhibitory loop.
Multiple transcript variants encoding different isoforms have been identified for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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