Thermo Fisher Scientific POLR3A Polyclonal Antibody
Human POLR3A를 인식하는 Rabbit Polyclonal Antibody로, WB, IHC, ICC/IF, IP 등 다양한 응용에 적합. 항원 친화 크로마토그래피로 정제되었으며, PBS 및 40% glycerol buffer에 보존. 연구용으로만 사용 가능.
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Thermo Fisher Scientific POLR3A Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL | View 1 publication |
| Immunohistochemistry (IHC) | – | View 1 publication |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL | – |
| Immunoprecipitation (IP) | – | View 1 publication |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human POLR3A. Recombinant protein control fragment (Product #RP-97106). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2645765 |
Product Specific Information
Immunogen sequence:
PSDDNPVRAN LRTKGKQYCG KGEDLCANDS YVTIQNSELM SGSMDKGTLG SGSKNNIFYI LLRDWGQNEA ADAMSRLARL APVYLSNRGF SI
Highest antigen sequence identity to orthologs:
- Mouse: 98%
- Rat: 98%
Target Information
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
POLR3A is the largest and catalytic core component of RNA polymerase III, which synthesizes small RNAs such as 5S rRNA and tRNAs.
It forms the polymerase active center together with the second largest subunit.
A single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III.
A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site, promoting translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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