Thermo Fisher Scientific POLR3A Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.04-0.4 µg/mL

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Immunohistochemistry (IHC)

-

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Immunocytochemistry (ICC/IF)

0.25-2 µg/mL

-

Immunoprecipitation (IP)

-

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Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

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View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2645765

Product Specific Information

Immunogen sequence: PSDDNPVRAN LRTKGKQYCG KGEDLCANDS YVTIQNSELM SGSMDKGTLG SGSKNNIFYI LLRDWGQNEA ADAMSRLARL APVYLSNRGF SI

Highest antigen sequence identity to the following orthologs: Mouse - 98%, Rat - 98%.

Target Information

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. POLR3A is the largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. It forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.