Thermo Fisher Scientific TCP1 Monoclonal Antibody (23c)
TCP1 단백질을 인식하는 Rat monoclonal antibody (clone 23c). Western blot, ICC/IF, IP에 사용 가능하며, 다양한 종에 반응. 단백질 접힘 관련 연구에 적합. PBS 용액 형태로 제공되며, 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 2–5 µg/mL | - |
| Immunocytochemistry (ICC/IF) | 5 µg/mL | - |
| Immunoprecipitation (IP) | 5 µg/mL | - |
| Miscellaneous PubMed (Misc) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Bovine, Dog, Hamster, Mouse, Sheep, Rabbit, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Rat / IgG2c |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 23c |
| Immunogen | Purified recombinant mouse TCP1 alpha construct encoding the C-terminal half of the 1.8 kb full-length Tcp1b gene expressed in E. coli. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7.2 |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2202549 |
Product Specific Information
Recommended positive controls: RK13 heat-shocked cell lysate.
Target Information
The T Complex Polypeptide 1 (TCP-1) is an approximately 60 kDa protein constitutively expressed in almost all eukaryotic cells and upregulated during spermatogenesis. It is found in the cytosol as a subunit of a hetero-oligomeric chaperone involved in the folding of actin and tubulin.
Chaperonins recognize and stabilize polypeptide intermediates during folding, assembly, and disassembly. They share similarities with Heat Shock Protein 70 (HSP70), including high abundance, stress induction, and ATPase activity. The chaperonin family includes mitochondrial HSP60, E. coli GroEL, plastid Rubisco-subunit binding protein, and archaebacterial TF55. The TCP-1 sequence shows nearly 40% identity to TF55, but minimal similarity to HSP60 and GroEL.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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