Thermo Fisher Scientific TRIM28 Recombinant Rabbit Monoclonal Antibody (ARC0047)
TRIM28 단백질을 인식하는 토끼 단클론 항체로, Western blot, IHC, ELISA, IP 등 다양한 응용에 적합합니다. 인간, 생쥐, 랫드 시료에 반응하며, HEK293 세포에서 발현된 재조합 항체입니다. 고순도의 Affinity Chromatography 정제 제품으로 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:6,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:2,000 |
| ELISA | 1 µg/mL |
| Immunoprecipitation (IP) | 0.5 µg–4 µg antibody for 200 µg–400 µg extracts |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | ARC0047 |
| Immunogen | Synthetic peptide corresponding to amino acids 250–350 of human KAP1/TRIM28 (Q13263) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.4 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol, 0.05% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | −20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2849205 |
Product Specific Information
Immunogen sequence:
RNQRKLLASL VKRLGDKHAT LQKSTKEVRS SIRQVSDVQK RVQVDVKMAI LQIMKELNKR GRVLVNDAQK VTEGQQERLE RQHWTMTKIQ KHQEHILRFA S
Target Information
The KRAB (Kruppel-associated box) domain is a ~45 amino acid transcriptional repression domain found in numerous transcription factors. Over 220 KRAB-zinc finger protein (KRAB-ZFP) genes have been identified in the human genome. These proteins repress transcription through interactions with KAP-1 (KRAB-associated protein 1), an 835 amino acid polypeptide containing a RING finger, B boxes, and a PHD finger.
KAP-1 forms complexes with KRAB-domain transcription factors, enhancing repression efficiency. It also interacts with HP1 (heterochromatin protein 1) and KRAZ1 (Kruppel-associated box-containing zinc finger protein 1), targeting KRAZ1 to centromeric heterochromatin foci containing HP1α, thereby regulating transcriptional repression. Mutations in KAP-1 that disrupt HP1 binding lead to random KRAZ1 distribution and strong transcriptional activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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