Thermo Fisher Scientific HIF-2 alpha Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 12 publications 12 publications

Immunohistochemistry (IHC)

-

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

1:100

-

Immunocytochemistry (ICC/IF)

1:100

-

Flow Cytometry (Flow)

Assay-Dependent

-

ELISA (ELISA)

1:100-1:2,000

-

Immunoprecipitation (IP)

5 µg/1 mg lysate

-

ChIP assay (ChIP)

1:10-1:500

-

Gel Shift (GS)

Assay-Dependent

-

In vitro Assay (IV)

Assay-Dependent

-

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Fish, Hamster, Human, Mouse, Non-human primate, Sheep, Rabbit, Reptile, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A peptide derived from the C-terminus of mouse/human HIF-2 alpha protein. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:HIF-2 alpha,uniProtId:Q99814-1,ncbiNodeId:9606,antigenRange:870,antigenLength:870,antigenImageFileName:PA1-16510_HIF-2_alpha_Q99814-1_Rabbit.svg,antigenImageFileNamePDP:PA1-16510_HIF-2_alpha_Q99814-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2098236

Product Specific Information

Suggested positive control: Cos7 CoCl2-treated nuclear extract, PC12 nuclear extracts.

Target Information

HIF-2 alpha (Epas1) is a transcription factor involved in the induction of genes regulated by oxygen. HIF-2 alpha contains a basic-helix-loop-helix domain protein dimerization domain as well as a domain found in proteins in signal transduction pathways which respond to oxygen levels. It also regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of the lungs. Mutations in this gene are associated with erythrocytosis familial type 4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.