
Thermo Fisher Scientific HIF-2 alpha Polyclonal Antibody
다양한 종에서 반응하는 HIF-2 alpha 폴리클로날 항체로, WB, IHC, ELISA 등 여러 응용 실험에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, PBS buffer에 0.05% sodium azide를 포함합니다. 연구용으로만 사용 가능합니다.
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Thermo Fisher Scientific HIF-2 alpha Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1–2 µg/mL | 12 publications |
| Immunohistochemistry (IHC) | - | 3 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 | 2 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100 | - |
| Immunocytochemistry (ICC/IF) | 1:100 | - |
| Flow Cytometry (Flow) | Assay-dependent | - |
| ELISA | 1:100–1:2,000 | - |
| Immunoprecipitation (IP) | 5 µg/1 mg lysate | - |
| ChIP assay (ChIP) | 1:10–1:500 | - |
| Gel Shift (GS) | Assay-dependent | - |
| In vitro Assay (IV) | Assay-dependent | - |
| Miscellaneous PubMed (Misc) | - | 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Fish, Hamster, Human, Mouse, Non-human primate, Sheep, Rabbit, Reptile, Rat |
| Published Species | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide derived from the C-terminus of mouse/human HIF-2 alpha protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2098236 |
Product Specific Information
Suggested positive control: Cos7 CoCl₂-treated nuclear extract, PC12 nuclear extracts.
Target Information
HIF-2 alpha (Epas1) is a transcription factor involved in oxygen-regulated gene expression. It contains a basic-helix-loop-helix domain and a dimerization domain, and is involved in signal transduction pathways responsive to oxygen levels. HIF-2 alpha regulates VEGF expression and plays a role in blood vessel and lung tubular system development. Mutations in this gene are associated with erythrocytosis familial type 4.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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