Thermo Fisher Scientific CD41a Monoclonal Antibody (HIP8), NovaFluor Blue 585, eBioscience

Applications

• Flow Cytometry (Flow): Tested at 5 µL (0.6 µg)/test

Product Specifications

Additional Available Formats

• Unconjugated
• APC
• APC-eFluor 780
• eFluor 450
• FITC
• NovaFluor Blue 510
• NovaFluor Blue 660-120S
• NovaFluor Yellow 590
• NovaFluor Yellow 660
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Custom conjugation available

Product Specific Information

The HIP8 monoclonal antibody reacts with human CD41 (integrin αIIb, platelet GPIIb). CD41 associates with integrin β3 (GPIIIa, CD61) and is expressed by megakaryocytes and platelets. The CD41/CD61 complex serves as a receptor for fibronectin, fibrinogen, von Willebrand factor, vitronectin, and thrombospondin, mediating platelet aggregation. HIP8 blocks platelet aggregation.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-titrated for flow cytometric analysis of normal human peripheral blood cells.
Use 5 µL (0.6 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for compatibility.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining with NovaFluor conjugates.
• Add 5 µL of CellBlox Plus per sample before combining with cells.
• For single-color controls, use 5 µL per 100 µL of cell sample.

Technology:
NovaFluor conjugates utilize Phiton™ technology with engineered nucleic acid dye structures to minimize spillover and spread in multicolor flow cytometry.

Excitation: 494 nm
Emission: 585 nm
Laser: 488 nm (Blue)

Target Information

CD41 (platelet glycoprotein IIb, ITGA2B) consists of two subunits (α: 120 kDa, β: 23 kDa) that interact with CD61 in the presence of calcium to form a functional adhesive receptor. It mediates platelet aggregation and binds von Willebrand factor, fibrinogen, fibronectin, and vitronectin upon vascular injury. CD41 is mainly expressed in the megakaryocyte-platelet lineage and during early hematopoietic differentiation.
Associated disorders include Glanzmann Thrombasthenia and Platelet type-16 bleeding disorder.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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