Thermo Fisher Scientific E2F4 Monoclonal Antibody (5B7)
인간 E2F4 단백질을 인식하는 Mouse IgG1 단일클론 항체로 Western blot 및 ELISA에 적합. GST 태그가 결합된 재조합 E2F4 단백질로 면역화됨. PBS 용액 형태로 제공되며 보존제 무첨가. -20°C에서 보관 필요.
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Applications
Western Blot (WB)
- Tested Dilution: 1–5 µg/mL
ELISA
- Tested Dilution: 0.03 ng/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5B7 |
| Immunogen | E2F4 (NP_001941, 211–300 a.a.) partial recombinant protein with GST tag (MW of GST tag: 26 kDa) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | See Label |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Sequence of this protein is as follows:
PPEDLLQSPS AVSTPPPLPK PALAQSQEAS RPNSPQLTPT AVPGSAEVQG MAGPAAEITV SGGPGTDSKD SGELSSLPLG PTTLDTRPLQ
Target Information
E2F transcription factors are functionally regulated by binding to Rb p110, p107, and p130.
E2F-4 is regulated by complex formation with Rb p110.
E2F family members bind DNA as heterodimers with members of the DP family of polypeptides.
Differential phosphorylation contributes to the microheterogeneity of E2F-4 (total aa 411–416, varies due to a trinucleotide repeat).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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