Thermo Fisher Scientific Nucleostemin Recombinant Rabbit Monoclonal Antibody (3H20L2)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:5,000

-

Immunocytochemistry (ICC/IF)

1:100-1:1,000

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

3H20L2

Immunogen

Recombinant protein corresponding to amino acids 1-180 of human Nucleostemin if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Nucleostemin,uniProtId:Q9BVP2-1,ncbiNodeId:9606,antigenRange:1-180,antigenLength:549,antigenImageFileName:701157_Nucleostemin_Q9BVP2-1_Rabbit.svg,antigenImageFileNamePDP:701157_Nucleostemin_Q9BVP2-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2532414

Product Specific Information

This antibody is predicted to react with equine, non-human primate, mouse, rabbit and rat based on sequence homology.

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

Arylsulfatase F, also known as ARSF, is a 590 amino acid secretory protein that belongs to the sulfatase family of bone and cartilage matrix proteins. Arylsulfatase F uses calcium as a cofactor to catalyze reactions that are important in maintaining correct bone composition. The activity of Arylsulfatase F, unlike that of other family members, such as Arylsulfatase E, is not inhibited by warfarin. The gene encoding Arylsulfatase F maps to human chromosome X, which contains nearly 153 million base pairs and houses over 1,000 genes. In conjunction with chromosome Y, chromosome X is responsible for sex determination. There are a number of conditions related to an abnormal number and combination of sex chromosomes, some of which include Turner`s syndrome, color blindness, hemophilia and Duchenne muscular dystrophy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.