Thermo Fisher Scientific CD25 Chimeric Recombinant Rat Monoclonal Antibody (PC-61.5.3)
쥐 단클론 항체로서 마우스 CD25(IL-2Rα)에 특이적 결합. 다양한 응용(WB, ICC/IF, Flow, ELISA, Functional assay)에 사용 가능. 재조합 항체로 높은 재현성과 특이성 제공. 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
| Flow Cytometry (Flow) | Assay-dependent |
| ELISA | Assay-dependent |
| Functional Assay | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Rat / IgG2a, lambda |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | PC-61.5.3 |
| Immunogen | This antibody was raised by immunising rats with the B6.1 mouse cytotoxic T cell line, followed by fusing spleen cells with P3X63Ag8.653 myeloma cells. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_3074613 |
Product Specific Information
Specificity:
This antibody is specific for murine CD25, the 55 kDa interleukin-2 receptor alpha chain (IL-2Rα). CD25 is expressed by early progenitors of the T and B lineage as well as by activated mature T and B lymphocytes. CD25 alone binds IL-2 with low affinity, but when associated with CD122 (IL-2 receptor β chain) and CD132 (common γ chain), it forms the high-affinity IL-2 receptor. Binding of IL-2 to both high- and low-affinity receptor classes is inhibited by the PC61.5 antibody.
Target Information
CD25 (IL2 receptor alpha chain/IL2RA) is a cytokine receptor involved in the proliferation of T and B lymphocytes. The IL2 receptor complex consists of IL2RA, IL2RB, and IL2RG chains forming a high-affinity receptor.
- Homodimeric IL2RA: low-affinity receptor
- Homodimeric IL2RB: medium-affinity receptor
IL2 is primarily produced by mature T cells and functions as a growth, differentiation, and apoptosis-regulating factor. It stimulates B cell proliferation, enhances NK cell activity, and inhibits granulocyte macrophage colony formation. Disruption of this gene in mice leads to ulcerative colitis-like disease, indicating its role in immune regulation.
Mutations in IL2RA are associated with interleukin-2 receptor alpha deficiency.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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