Thermo Fisher Scientific PION Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.25 µg/mL

View 1 publication 1 publication

Immunohistochemistry (IHC)

5 µg/mL

-

Immunocytochemistry (ICC/IF)

20 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A 19 amino acid peptide near the carboxy terminus of human PION. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PION,uniProtId:A4D1B5-1,ncbiNodeId:9606,antigenRange:854,antigenLength:854,antigenImageFileName:PA5-21092_PION_A4D1B5-1_Rabbit.svg,antigenImageFileNamePDP:PA5-21092_PION_A4D1B5-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11153200

Product Specific Information

A suggested positive control is EL4 cell lysate.

PA5-21092 can be used with blocking peptide PEP-1206.

Target Information

Accumulation of the amyloid-beta peptide (Abeta) in the cerebral cortex is a critical event in the pathogenesis of Alzheimers disease. The beta-amyloid protein precursor (APP) is cleaved by one of two beta-secretases (BACE and BACE2), producing a soluble derivative of the protein and a membrane anchored 99 -amino acid carboxy-terminal fragment (C99). The C99 fragment serves as substrate for -gamma-secretase to generate the 4 kDa amyloid-beta peptide (Abeta), which is deposited in the Alzheimers disease patientsbrains. PION, or GSAP, selectively increases amyloid-beta production through a mechanism involving its interaction with both -gamma-secretase and the APP C-terminal fragment, suggesting that PION may be a potential therapeutic target for the treatment of Alzheimers disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.