Thermo Fisher Scientific SPAG5 Monoclonal Antibody (OTI2F2), TrueMAB
SPAG5 단백질을 인식하는 Mouse IgG2a 단일클론 항체로 Western blot에 최적화됨. 인간 SPAG5(aa 1–297) 재조합 단백질로 면역화. Lyophilized 형태, PBS buffer에 trehalose 포함. 연구용으로만 사용 가능.
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Applications
- Western Blot (WB): 1:500–1:2,000 dilution
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2F2 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1–297 of human SPAG5 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform additional desalting using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
SPAG5 (sperm associated antigen 5), also known as MAP126, hMAP126, DEEPEST, or Astrin, is a 1,193 amino acid protein highly expressed in testis and moderately in liver, placenta, pancreas, colon, and thymus.
It localizes to spindle poles during prophase and throughout the spindle during metaphase and anaphase, playing an essential role in spindle formation and mitotic regulation.
SPAG5 is involved in sister chromatid cohesion and separation, maintaining chromosome integrity through homodimer formation with a long stalk and globular head domain.
Upon DNA damage, SPAG5 may be phosphorylated by ATR or ATM.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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