Thermo Fisher Scientific HUS1B Polyclonal Antibody
HUS1B 단백질에 특이적인 Thermo Fisher Scientific의 토끼 폴리클로날 항체. Western blot, ELISA, Immunoprecipitation에 사용 가능. 고순도 친화성 정제 항체로 DNA 손상 반응 연구에 적합. -20°C 보관, 반복 동결·해동 방지 필요.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| ELISA | 1:20,000–1:100,000 |
| Immunoprecipitation (IP) | 1:500–1:5,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to an internal region near amino acids 100–125 of human Hus1B protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.75 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at -20°C prior to opening.
- Aliquot contents and freeze at -20°C or below for extended storage.
- Avoid cycles of freezing and thawing.
- Centrifuge product if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
This affinity-purified antibody is directed against human Hus1B protein.
A BLAST analysis indicates 100% homology of the immunizing sequence with Hus1B protein from human.
Partial reactivity with mouse Hus1B is not likely based on low (~64%, 9/14) sequence homology.
Reactivity against homologues from other sources is not known.
Target Information
HUS1B is closely related to HUS1, a component of a cell cycle checkpoint protein complex involved in cell cycle arrest in response to DNA damage.
HUS1B can interact with the checkpoint protein RAD1 but not with RAD9.
Overexpression of HUS1B has been shown to induce cell death, suggesting a distinct role compared to HUS1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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