Thermo Fisher Scientific IL-6 Monoclonal Antibody (MP5-20F3), Functional Grade, eBioscience

Thermo Fisher Scientific IL-6 Monoclonal Antibody (MP5-20F3), Functional Grade, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Brilliant Violet 650
• eFluor 450
• FITC
• PE
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The MP5-20F3 antibody reacts with mouse interleukin-6 (IL-6) and functions as a neutralizing antibody. Mouse IL-6 (also known as B Cell Stimulating Factor, BCSF, and BSF-2) is a 21–28 kDa cytokine produced by various cell types, including monocytes, fibroblasts, and endothelial cells. It influences immune and inflammatory responses and plays a role in neuronal development and acute phase reactions.

Applications Reported:

• Cytokine neutralization
• ELISA capture antibody
• ELISPOT capture antibody

Applications Tested:
Functional Grade Purified MP5-20F3 antibody was tested by LAL assay (low endotoxin), ELISA, and bioassay for IL-6 neutralization.
At 0.03 µg/mL, it inhibited by 50% the biological effects of 0.01 ng/mL mouse IL-6 in a B9 cell proliferation assay.
Detailed protocols are available in the BestProtocols® section.

ELISA Use:
Tested as a capture antibody for mouse IL-6 in sandwich ELISA with biotinylated MP5-32C11 (Product # 13-7062-81) and recombinant IL-6 ([Product # 14-8061]) as the standard.
Recommended concentration range: 0.5–2 µg/mL.
Standard curve range: 8–1000 pg/mL.

Storage and Handling:
Use in sterile environment.

Filtration:
0.2 µm post-manufacturing filtered.

Purity:

90% (SDS-PAGE)

Endotoxin Level:
<0.001 ng/µg antibody (LAL assay)

Aggregation:
<10% (HPLC)

Target Information

IL-6 encodes a cytokine involved in inflammation and B cell maturation. It acts as an endogenous pyrogen, inducing fever in autoimmune or infectious conditions. Expressed at inflammation sites, IL-6 triggers transcriptional responses via IL-6 receptor alpha. Elevated IL-6 levels are associated with inflammatory diseases, diabetes mellitus, systemic juvenile rheumatoid arthritis, and viral infections including COVID-19. Related diseases include Kaposi Sarcoma and Rheumatoid Arthritis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.