
Thermo Fisher Scientific Alkaline Phosphatase Polyclonal Antibody
Thermo Fisher의 Alkaline Phosphatase Polyclonal Antibody는 송아지 장 유래 항원을 이용한 염기성 인산분해효소 검출용 항체입니다. Western blot, ELISA, IP에 적합하며, 비접합 형태로 제공됩니다. 장기 보관 시 -20°C에서 안정적으로 유지됩니다.
- 카탈로그번호
- 100-1135
- 판매단위
- pk
카탈로그
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| ELISA | 1:4,000–1:20,000 |
| Immunoprecipitation (IP) | 1:500–1:2,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bovine |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Alkaline Phosphatase (Calf intestine) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 25 mg/mL |
| Storage Buffer | 0.01M sodium phosphate/whole serum, pH 7.2, with 0.15M NaCl |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- For extended storage, aliquot contents and freeze at -20°C or below.
- Avoid cycles of freezing and thawing.
- Centrifuge product if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, purified and partially purified Alkaline Phosphatase (Calf intestine). Cross-reactivity against Alkaline Phosphatase from other tissues and species may occur but has not been specifically determined. No reaction was observed against the bacterial form of the enzyme.
Target Information
Alkaline phosphatase (ALP) is a homodimeric enzyme (86 kDa) containing two zinc atoms per monomer essential for catalytic function, optimally active at alkaline pH.
It is found in both prokaryotes and eukaryotes, maintaining similar functions but structurally adapted to each organism’s environment.
ALP plays a key role in liver metabolism and skeletal development. In bacteria, ALP provides inorganic phosphate, and in yeast, it serves as a useful protein for studying membrane protein sorting.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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