Thermo Fisher Scientific E4F1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.04-0.4 µg/mL

-

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL

-

Immunoprecipitation (IP)

1:25

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant Human E4F1. Recombinant protein control fragment (Product #RP-105531). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:E4F1,uniProtId:Q66K89-1,ncbiNodeId:9606,antigenRange:265-339,antigenLength:784,antigenImageFileName:PA5-67167_E4F1_Q66K89-1_Rabbit.svg,antigenImageFileNamePDP:PA5-67167_E4F1_Q66K89-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2663632

Product Specific Information

Immunogen sequence: RHLKSLTPCTE KIRFSVSKDV VVSKEDARAG SGAGAAGLGT ATSSVTGEPI ETSPVIHLVT DAKGTVIHEV HVQM

Highest antigen sequence identity to the following orthologs - mouse 77%, rat 79%.

Target Information

May function as a transcriptional repressor. May also function as a ubiquitin ligase mediating ubiquitination of chromatin-associated TP53. Functions in cell survival and proliferation through control of the cell cycle. Functions in the p53 and pRB tumor suppressor pathways and regulates the cyclin CCNA2 transcription.; FUNCTION: Identified as a cellular target of the adenoviral oncoprotein E1A, it is required for both transcriptional activation and repression of viral genes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.