Thermo Fisher Scientific Desmin Polyclonal Antibody
Desmin 단백질을 인식하는 토끼 폴리클로날 항체로, WB, IHC, ICC 등 다양한 응용에 사용 가능. 인간, 마우스, 랫 등 여러 종에 반응. 항원 친화 크로마토그래피로 정제되었으며, 장기 보관 시 -20°C 권장. 연구용 전용 제품.
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Applications and Tested Dilution
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:500–1:10,000 | |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 | |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-dependent | |
| Immunocytochemistry (ICC/IF) | Assay-dependent | |
| Flow Cytometry (Flow) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chicken, Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Desmin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.42 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2792333 |
Product Specific Information
- Keep as concentrated solution.
- Predicted reactivity: Mouse (98%), Rat (99%), Dog (99%), Pig (99%), Rabbit (99%), Rhesus Monkey (99%), Bovine (99%).
- Positive Control: RD, mouse muscle.
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Desmin (approximately 53 kDa) exhibits a high degree of tissue specificity, being predominantly expressed in all types of muscle cells (cardiac, skeletal, and smooth muscle). Regulation of desmin expression is stage- and tissue-specific, induced during terminal development such as skeletal muscle differentiation.
In skeletal and cardiac muscle cells, desmin is localized in the Z-disk region and at the intercalated disk. Expression in smooth muscle is more heterogeneous.
Coexpression of vimentin and desmin has been observed in tumors derived from muscle tissue such as rhabdomyosarcomas and leiomyosarcomas.
During myocardial dysfunction, dramatic changes in desmin distribution have been observed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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