Thermo Fisher Scientific tPA Polyclonal Antibody
Thermo Fisher Scientific의 tPA Polyclonal Antibody는 인간, 마우스, 랫트 반응성을 가지며 Western blot과 IHC에 적합합니다. 항원 친화 크로마토그래피로 정제된 고품질 항체로, 조직 재형성 및 세포 이동 관련 연구에 활용됩니다. 장기 보관 시 -20°C에서 안정적으로 유지됩니다.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:3,000
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:100–1:1,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human tPA |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.15 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol, 1% BSA |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2792332 |
Product Specific Information
- Keep as concentrated solution.
- Predicted reactivity: Mouse (80%), Rat (82%), Pig (83%), Bovine (82%).
- Positive Control: 293T, Neuro2A, PC-12, human plasma.
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
TPA converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, cell migration, and other physiological and pathological events.
TPA specifically cleaves the Arg-Val bond in plasminogen to form plasmin, and is comprised of a heterodimer of chain A and chain B held by a disulfide bond.
It binds to fibrin with high affinity, increasing catalytic efficiency 100–1000 fold due to enhanced affinity for plasminogen. Binding to heparin, laminin, and fibronectin also enhances activation.
TPA interacts with mannose receptor and LRP1, which are involved in clearance, and binds to annexin II and cytokeratin 8.
Interactions on endothelial and vascular smooth muscle cells (VSMC) stimulate plasminogen activation 100-fold and reduce inhibition by PAI-1 by 30-fold.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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