Thermo Fisher Scientific A33 Recombinant Rabbit Monoclonal Antibody (9W5A7)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

9W5A7

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 220-319 of human GPA33 (Q99795). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:A33,uniProtId:Q99795-1,ncbiNodeId:9606,antigenRange:220-319,antigenLength:319,antigenImageFileName:MA5-42830_A33_Q99795-1_Rabbit.svg,antigenImageFileNamePDP:MA5-42830_A33_Q99795-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.23 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 0.05% BSA, 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2911971

Product Specific Information

Positive test controls include: HCT116, HT-29, A-549, Mouse stomach, Rat large intestine. The target is usually found in the following locations: Membrane, Single-pass type I membrane protein.

Immunogen sequence: QFCNITVAVR SPSMNVALYV GIAVGVVAAL IIIGIIIYCC CCRGKDDNTE DKEDARPNRE AYEEPPEQLR ELSREREEED DYRQEEQRST GRESPDHLDQ

Target Information

The glycoprotein encoded by this gene is a cell surface antigen that is expressed in greater than 95% of human colon cancers. The open reading frame encodes a 319-amino acid polypeptide having a putative secretory signal sequence and 3 potential glycosylation sites. The predicted mature protein has a 213-amino acid extracellular region, a single transmembrane domain, and a 62-amino acid intracellular tail. The sequence of the extracellular region contains 2 domains characteristic of the CD2 subgroup of the immunoglobulin (Ig) superfamily.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.