Thermo Fisher Scientific A33 Recombinant Rabbit Monoclonal Antibody (9W5A7)
A33 단백질을 표적으로 하는 재조합 래빗 모노클로날 항체로, WB, IHC, ICC, ELISA 등 다양한 응용에 적합. HEK293 세포에서 발현된 IgG 형 항체이며, 인간·마우스·랫트 반응성. 고순도 친화 크로마토그래피 정제 및 안정적 액상 형태로 제공.
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Thermo Fisher Scientific A33 Recombinant Rabbit Monoclonal Antibody (9W5A7)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 9W5A7 |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 220–319 of human GPA33 (Q99795) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.23 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 0.05% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | –20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2911971 |
Product Specific Information
- Positive test controls: HCT116, HT-29, A-549, Mouse stomach, Rat large intestine
- Localization: Membrane, Single-pass type I membrane protein
- Immunogen sequence:
QFCNITVAVR SPSMNVALYV GIAVGVVAAL IIIGIIIYCC CCRGKDDNTE DKEDARPNRE AYEEPPEQLR ELSREREEED DYRQEEQRST GRESPDHLDQ
Target Information
The glycoprotein encoded by this gene is a cell surface antigen expressed in over 95% of human colon cancers.
It encodes a 319-amino acid polypeptide with a secretory signal sequence and three potential glycosylation sites.
The mature protein consists of a 213-amino acid extracellular region, a single transmembrane domain, and a 62-amino acid intracellular tail.
The extracellular region contains two domains characteristic of the CD2 subgroup of the immunoglobulin superfamily.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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