Thermo Fisher Scientific GAPDH Monoclonal Antibody (OTI2F8), TrueMAB
인간 GAPDH를 인식하는 Mouse IgG1 단일클론 항체로 Western blot 및 IHC(P) 실험에 적합. 고순도 친화 크로마토그래피 정제 제품으로, carrier-free 형태로 제공되어 결합 실험에 활용 가능. 세포 내 GAPDH 검출 및 로딩 컨트롤용으로 이상적.
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Thermo Fisher Scientific GAPDH Monoclonal Antibody (OTI2F8), TrueMAB
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2F8 |
| Immunogen | Full length human recombinant GAPDH protein produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is a catalytic enzyme involved in glycolysis, forming a tetramer of identical 37-kDa subunits. It catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphate in the presence of NADPH.
Beyond glycolysis, GAPDH participates in DNA replication, repair, nuclear RNA export, membrane fusion, and microtubule bundling. It plays roles in gene expression during apoptosis and is implicated in neurodegenerative diseases such as Alzheimer’s and Huntington’s.
Due to its high expression in most tissues, GAPDH serves as a reliable protein loading control in Western blot analysis.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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