Thermo Fisher Scientific SMAD4 Recombinant Rabbit Monoclonal Antibody (PD01-37)
SMAD4 단백질을 인식하는 재조합 토끼 단클론 항체로 Western blot, IHC, ICC/IF에 사용 가능. 인간, 마우스, 랫트 반응성. HEK293 세포 발현, Protein A 정제, 고순도 액상 형태. 세포 내 SMAD4 위치 및 발현 연구에 적합.
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Thermo Fisher Scientific SMAD4 Recombinant Rabbit Monoclonal Antibody (PD01-37)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:50 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | PD01-37 |
| Immunogen | Synthetic peptide within Human Smad4 aa 500–552 (C-terminal) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4, with 0.1% BSA, 40% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_3094155 |
Product Specific Information
- Positive control: PC-12 cell lysate, SH-SY5Y cell lysate, human lung carcinoma tissue, human testis tissue, human stomach carcinoma tissue, PANC-1
- Predicted band size: 60 kDa
- Subcellular Location: Cytoplasm, Nucleus
Target Information
Smad4 is encoded by the Smad4 gene located on chromosome 18 in humans. It is also known as Smad family member 4, Sma- and Mad-related protein 4, or Mothers against decapentaplegic (MAD) homolog 4.
Smad4 contains N-terminal MH1 (MAD homology 1) and C-terminal MH2 (MAD homology 2) domains involved in DNA binding and protein interactions.
Binding of TGF-beta superfamily ligands (including TGF-beta and BMP) to their receptors leads to phosphorylation of Smad1, 2, 3, 5, and 8 (R-Smads), which then form complexes with Smad4 (co-Smad) and translocate to the nucleus.
Smad4 acts as a tumor suppressor by regulating target genes such as Cyclin D1 (downregulation) and collagen (upregulation), thereby inhibiting cell proliferation.
Dephosphorylation regulates nuclear export and nucleocytoplasmic dynamics of Smads.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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