Thermo Fisher Scientific POLR2H Monoclonal Antibody (OTI6E10), TrueMAB
POLR2H 단백질을 인식하는 Mouse Monoclonal Antibody (Clone OTI6E10). Western blot에 최적화되어 있으며 Human, Mouse, Rat에 반응. Lyophilized 형태로 제공되며, PBS buffer와 trehalose 포함. 연구용으로 RNA polymerase II 관련 연구에 적합.
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Applications
- Western Blot (WB): 1:500–1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI6E10 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 2–150 of human POLR2H produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
Eukaryotic cells produce three distinct classes of RNA polymerase: Pol I, II, and III.
- Pol I: Transcribes rRNA genes for the 28S, 18S, and 5.8S ribosomal RNA precursors.
- Pol II: Transcribes protein-coding genes into mRNA and snRNA genes into snRNAs that influence RNA processing.
- Pol III: Transcribes 5S rRNA and all tRNA genes.
Each RNA polymerase complex comprises 9–15 polypeptides, with RPB6 and RPB8 subunits shared among all three.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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