Thermo Fisher Scientific LAMP1 Monoclonal Antibody (H4A3)

Applications and Tested Dilutions

Product Specifications

Available Conjugated Formats

• Alexa Fluor 488 (MA5-18121)
• APC (A15729)
• PE (A15798)
• Custom conjugation available upon request

Product Specific Information

This antibody recognizes an extracellular/luminal epitope of CD107a, an approximately 100–120 kDa glycoprotein expressed mainly on lysosomal membranes and also on the plasma membrane.

Target Information

LAMP1 (CD107a, lysosome-associated membrane protein-1) is a major component of the lysosomal membrane, with 1–2% also present on the plasma membrane. It is a heavily glycosylated type I integral membrane protein containing a signal peptide, 18 N-linked glycosylation sites, a transmembrane domain, and a short cytosolic tail.

LAMP1 and LAMP2 are involved in lysosome biogenesis and fusion with phagosomes. Upon cell activation, LAMP1 translocates to the plasma membrane via a tyrosine-based motif (YXXI). Mutations disrupting this motif alter its localization between lysosomes and the plasma membrane.

Cell surface LAMP1 promotes adhesion of PBMCs to vascular endothelium, suggesting a role in inflammation. Increased LAMP1 expression is observed in neurons and glial cells near senile plaques in Alzheimer’s disease and in macrophages and lymphocytes during thymic involution. LAMP1 is a useful marker for mast cell activation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.