Thermo Fisher Scientific Cdc25C Polyclonal Antibody
Rabbit polyclonal antibody against human CDC25C, validated for ICC/IF and ELISA. Recognizes residues 174–473 of CDC25C. Supplied as a liquid, unconjugated form in PBS with glycerol. Suitable for research on cell cycle regulation and mitotic control.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein corresponding to amino acids 174–473 of human CDC25C (NP_0017812) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.62 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2804661 |
Product Specific Information
Immunogen sequence:
PKLDKNPNLG EDQAEEISDE LMEFSLKDQE AKVSRSGLYR SPSMPENLNR PRLKQVEKFK DNTIPDKVKK KYFSGQGKLR KGLCLKKTVS LCDITITQML EEDSNQGHLI GDFSKVCALP TVSGKHQDLK YVNPETVAAL LSGKFQGLIE KFYVIDCRYP YEYLGGHIQG ALNLYSQEEL FNFFLKKPIV PLDTQKRIII VFHCEFSSER GPRMCRCLRE EDRSLNQYPA LYYPELYILK GGYRDFFPEY MELCEPQSYC PMHHQDHKTE LLRCRSQSKV QEGERQLREQ IALLVKDMSP
Positive Samples:
K-562, HepG2, Raji, HeLa, SW480, Mouse eye, Mouse spleen, Mouse intestine
Cellular Location:
Nucleus
Target Information
M-phase inducer phosphatase 3 (CDC25C) is an enzyme encoded by the CDC25C gene in humans. It plays a key role in regulating cell division by dephosphorylating cyclin B-bound CDC2 (CDK1), thereby triggering entry into mitosis. The protein belongs to the Cdc25 phosphatase family and may also suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants have been identified, though their full-length nature is not always known.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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