Thermo Fisher Scientific Vimentin Monoclonal Antibody (VIM, 3736)
Human Vimentin 단백질을 인식하는 Mouse monoclonal antibody로, Western blot 및 Immunohistochemistry에 적합합니다. Mouse와 Rat에는 반응하지 않으며, Protein A/G로 정제된 액상 형태입니다. 연구용으로만 사용 가능합니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | VIM, 3736 |
| Immunogen | Recombinant fragment (aa 2–466) of human Vimentin protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- This antibody does not react with Mouse or Rat.
- For Immunohistochemistry (PFA fixed): incubate antibody for 30 min at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 min at 95°C, followed by cooling at room temperature for 20 min.
Target Information
Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It plays a role in intracellular transport between the nucleus and plasma membrane and is co-expressed with desmin during early cellular development. Vimentin is later replaced by tissue-specific IFPs. It is implicated in steroid synthesis by serving as a storage network for cholesterol-containing lipid droplets. Phosphorylation of vimentin by protein kinase induces filament breakdown and cytoskeletal reorganization, facilitating cholesterol transport to mitochondria and enhancing steroid production.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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