Thermo Fisher Scientific XPF Monoclonal Antibody (51)

Applications

Tested Dilution

Publications

Western Blot (WB)

1-3 µg/mL

View 7 publications 7 publications

Immunocytochemistry (ICC/IF)

-

View 3 publications 3 publications

ChIP assay (ChIP)

1.5 µg/10^6 cells

-

Neutralization (Neu)

-

View 1 publication 1 publication

in situ PLA (PLA)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

51

Immunogen

Recombinant human XPF protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10987557

Product Specific Information

MA5-12060 targets XPF in WB applications and shows reactivity with Human samples.

The MA5-12060 immunogen is recombinant human XPF protein.

Target Information

The structure specific ERCC 1 / XPF endonuclease complex is implicated in the repair of two distinct types of lesions in DNA: NER for UV induced lesions and bulky chemical adducts; and recombination repair of the very genotoxic interstrand cross links. NER mechanism involves dual incisions on both sides of the damage catalyzed by two nucleases. In mammalian cells, XPG cleaves 3of the DNA lesion while the ERCC1 / XPF complex makes the 5 incision. ERCC1 / XPF probably plays a role in processing partially processed recombination intermediates.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.