Thermo Fisher Scientific GFAP Monoclonal Antibody (ASTRO6)

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 12 publications 12 publications

Immunohistochemistry (IHC)

-

View 48 publications 48 publications

Immunohistochemistry (Paraffin) (IHC (P))

1-2 µg/mL

View 5 publications 5 publications

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

1:20-1:200

View 17 publications 17 publications

Flow Cytometry (Flow)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Chicken, Human, Pig, Rat

Published species

Human, Mouse, Pig, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

ASTRO6

Immunogen

Glial Fibrillary Acidic Protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

200 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10984338

Product Specific Information

MA5-12023 targets Glial Fibrillary Acidic Protein in IF, IHC (P), and WB applications and shows reactivity with Chicken, Human, Porcine, and Rat samples.

The MA5-12023 immunogen is glial Fibrillary Acidic Protein.

MA5-12023 was successfully used to detect astrocytes in E18 Sparague Dawley primary cortical cells.

Target Information

GFAP (Glial fibrillary acidic protein) is a member of the class III intermediate filament protein family. GFAP is heavily and specifically expressed in astrocytes and certain astroglia of the central nervous system, in satellite cells of peripheral ganglia, and in non-myelinating Schwann cells of peripheral nerves. In addition, neural stem cells strongly express GFAP. Antibodies to GFAP are very useful as markers of astrocytic cells. In addition, many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes. GFAP is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing of the GFAP gene results in multiple transcript variants encoding distinct isoforms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.