Thermo Fisher Scientific Mitochondria Monoclonal Antibody (MTC02)
인간 미토콘드리아 60kDa 비당화 단백질을 인식하는 단클론 항체. Western blot, IHC, ICC 등 다양한 응용 가능. 포르말린 고정 조직 염색 시 시트르산 완충액 항원 회복 필요. 연구용으로 세포 내 미토콘드리아 마커로 활용.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1–2 µg/mL | View 3 publications |
| Immunohistochemistry (IHC) | – | View 9 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL | View 3 publications |
| Immunocytochemistry (ICC/IF) | 2 µg/mL | View 4 publications |
| Luminex (LUM) | – | View 1 publication |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MTC02 |
| Immunogen | Semi-purified mitochondrial preparation |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10983622 |
Product Specific Information
This antibody recognizes a 60 kDa non-glycosylated protein component of mitochondria found in normal and malignant human cells.
Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer (pH 6.0) for 10–20 minutes, followed by cooling at room temperature for 20 minutes.
Recommended positive controls: HeLa cells and liver tissue.
Target Information
Antibodies that recognize subcellular organelles or compartments of human cells are useful in identifying these organelles in cells, tissues, and biochemical preparations.
Anti-mitochondria antibodies can be used to stain mitochondria in cell or tissue preparations and serve as markers in subcellular fraction studies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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