Thermo Fisher Scientific IPO5 Polyclonal Antibody

Applications and Tested Dilution

• Immunohistochemistry (Paraffin) [IHC (P)]: 1:20–1:50

Product Specifications

Product Specific Information

• Immunogen sequence: MPEDQVGKLE ATENTISAMA
• Highest antigen sequence identity: Mouse - 45%, Rat - 45%

Target Information

Nucleocytoplasmic transport is a signal- and energy-dependent process occurring through nuclear pore complexes in the nuclear envelope. Import of proteins with a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits (karyopherins).
Importin alpha binds the NLS-containing cargo in the cytoplasm, and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP-binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein, while importin beta remains at the pore. Interactions between importin beta and FG repeats of nucleoporins are essential for translocation through the pore complex. The encoded protein belongs to the importin beta family.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.