Thermo Fisher Scientific Cortactin Polyclonal Antibody
Thermo Fisher Scientific의 Cortactin Polyclonal Antibody는 인간, 마우스, 랫트 시료에서 반응하며, WB, IHC, ICC/IF 등 다양한 응용에 적합합니다. 액상 형태로 1 mg/mL 농도이며, 항원 친화 크로마토그래피로 정제되었습니다. 세포 이동 및 액틴 구조 연구용으로 최적화된 항체입니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
| Flow Cytometry (Flow) | – |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to a region within amino acids 1–43 of Human Cortactin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2544610 |
Product Specific Information
- Recommended positive controls: HeLa, HepG2, NIH-3T3, JC, PC-12, mouse brain, rat brain
- Predicted reactivity: Rhesus Monkey (100%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Cortactin (Cttn) is a ubiquitous actin-binding protein originally identified as a substrate for Src. It contributes to actin cytoskeleton organization and cell shape, playing key roles in lamellipodia formation and cell migration. Cortactin accumulates in actin-enriched peripheral structures, facilitating actin network formation. It contains four major domains: N-terminal acidic (NTA), tandem repeats, proline-rich, and SH3 domains. The NTA domain associates with Arp2/3 and WASP complexes at F-actin branches. Cortactin promotes cell motility and invasion and is critical for invadopodia formation, where actin-rich protrusions degrade ECM in cancer cells. Phosphorylation occurs at Y421, Y466, Y482 by Src kinases and at S405, S418 by Erk kinases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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