Thermo Fisher Scientific Collagen Type IV Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

Immunohistochemistry (IHC)

1:100

-

Immunocytochemistry (ICC/IF)

15 µg/mL

-

ELISA (ELISA)

1:10,000-1:50,000

-

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Anti-Collagen IV antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 240-265 of mouse Collagen IV. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Collagen IV,uniProtId:P02463-1,ncbiNodeId:10090,antigenRange:240-265,antigenLength:1669,antigenImageFileName:600-101-MN4_Collagen_IV_P02463-1_Goat.svg,antigenImageFileNamePDP:600-101-MN4_Collagen_IV_P02463-1_Goat_PDP.jpeg,sortOrder:10}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.92 mg/mL

Purification

Affinity chromatography

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

This affinity purified antibody is directed against mouse Collagen IV. Blast analysis of the sequence of the immunogen shows 100% identity with mouse and 78.6% identity with human.

Target Information

Collagen IV is a type of collagen protein that is a major component of the basement membrane in various tissues, including the kidneys, lungs, and skin. It forms a network-like structure that provides support and stability to the surrounding tissue. Collagen IV is composed of three alpha chains, which are encoded by the COL4A1, COL4A2, and COL4A3 genes. Mutations in these genes can lead to various genetic disorders, such as Alport syndrome and Goodpasture syndrome, which affect the kidneys and lungs, respectively.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.