Norgen 2X One-Step RT-PCR Master Mix

Norgen 2X One-Step RT-PCR Master Mix

SKU: 28115

A ready-to-use solution optimized for robust RT-PCR amplification.

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Features and Benefits

• Convenient ready-to-use solution
• High sensitivity and yield
• Robust amplification
• Available in 3 convenient sizes: 100, 200, and 500 reactions (20 µL vol)
• Recommended for use with Norgen’s COVID-19 Primer and Probe Solutions and Positive Control

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Product Description

Norgen’s 2X One-Step RT-PCR Master Mix is a ready-to-use solution containing all components required for RT-PCR amplification of RNA templates. The mix includes M-MuLV reverse transcriptase, Taq DNA polymerase, dNTPs, reaction buffer, MgCl₂, KCl, and a PCR enhancer/stabilizer.
Users only need to add the template, primer set, and water. This mix minimizes setup time and contamination risk, especially for high-throughput applications, and ensures robust amplification with high yields.

Enzyme Details:

• Taq DNA Polymerase: Highly thermostable enzyme with 5′→3′ polymerase activity and low exonuclease activity, derived from Thermus aquaticus YT-1.
• M-MuLV Reverse Transcriptase: RNA-directed DNA polymerase capable of synthesizing cDNA from RNA or single-stranded DNA templates, derived from E. coli expressing the M-MuLV gene.

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Available Sizes

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Supporting Data

Figure 1. Successful Amplification of Different Sized Amplicons

OCR 추출 텍스트:
1 2 3 4 5 6 7 8 M
각 숫자는 젤 전기영동상의 샘플 라인, “M”은 마커(Marker)를 의미합니다.

Norgen’s 2X One-Step RT-PCR Master Mix was used to amplify various amplicons:

• Lanes 1–2: E. coli 16S rRNA (1.5 kb)
• Lanes 3–4: E. coli 16S rRNA (550 bp)
• Lanes 5–6: Human S15 gene (360 bp)
• Lanes 7–8: Human 5S rRNA (120 bp)
  All products were amplified with high sensitivity and yield.

Figure 2. Robust Amplification from Different Amounts of RNA Input

OCR 추출 텍스트:
M 1 2 3 4 5 6 7 8
M은 마커, 1~8은 RNA 입력량이 다른 샘플을 의미합니다.

The mix amplified human S15 transcripts from HeLa total RNA (ng to fg range), showing robust amplification across a wide RNA input range.

Figure 3. SARS-CoV-2 Detection

OCR 추출 텍스트:

• Amplification
• Norgen (Blue)
• Competitor N (Red)
• RFU vs Cycles

그래프 설명:
Norgen (Blue) shows higher sensitivity compared to Competitor N (Red) in SARS-CoV-2 detection using Norgen’s 2019-nCoV Primer & Probe Mixes and Positive Control.

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Reagents Supplied (Cat #28115)

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Storage Conditions and Stability

• Store at -20°C.
• For frequent use, aliquots can be stored at 4°C for up to 3 months.
• Avoid repeated freeze-thaw cycles.
• Stable for at least 1 year when properly stored.

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Documentation

Protocols

• 28113 - Protocol (100 rxns)
• 28114 - Protocol (200 rxns)
• 28115 - Protocol (500 rxns)

Application Notes

• Using Norgen’s Saliva/Swab RNA Purification Kit on the Hamilton Vantage to Isolate RNA from Fresh and Preserved Saliva Samples without Contamination

Safety Data Sheets

• 28113 - SDS
• 28114 - SDS
• 28115 - SDS

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Citations

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