Thermo Fisher Scientific Phospho-EGFR (Tyr1173) Polyclonal Antibody
EGFR 단백질의 Tyr1173 인산화 형태를 특이적으로 인식하는 폴리클로날 항체. Western blot 및 Immunocytochemistry에 적합. 항원 친화 크로마토그래피로 정제된 고품질 제품으로, 다양한 암 연구 및 신호전달 연구에 활용 가능.
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Thermo Fisher Scientific Phospho-EGFR (Tyr1173) Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Carrier-protein conjugated synthetic peptide corresponding to residues around human EGFR (phospho Tyr1173) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.17 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 1% BSA, 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C and avoid freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2792932 |
Product Specific Information
- Keep as a concentrated solution.
- Positive Control: A431 cells (starved for 16 hr and treated with 100 ng/mL EGF for 5 min).
- Centrifuge briefly prior to opening the vial.
Target Information
EGFR (epidermal growth factor receptor) is a receptor tyrosine kinase that mediates signaling in response to various growth factors. Overexpression of EGFR is associated with multiple cancer types and serves as a target for biological and small-molecule therapeutics.
EGFR is encoded by the EGFR gene located on chromosome 7 in humans and belongs to the HER/ErbB family, which includes ERbB2, ERbB3, and ERbB4.
Binding of ligands such as EGF and TGF-α to the extracellular domain promotes receptor dimerization and autophosphorylation of tyrosine residues in the cytoplasmic domain.
Overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, and squamous cell carcinoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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