Thermo Fisher Scientific CD163 Monoclonal Antibody (TNKUPJ), Super Bright 600, eBioscience

Thermo Fisher Scientific CD163 Monoclonal Antibody (TNKUPJ), Super Bright 600, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 532
• APC
• Brilliant UV 615
• Brilliant UV 661
• Brilliant UV 737
• Brilliant UV 805
• Brilliant Violet 421
• eFluor 450
• FITC
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 702
• Request custom conjugation

Product Specific Information

This TNKUPJ monoclonal antibody recognizes mouse CD163, a 130 kDa surface receptor expressed by specific subsets of tissue macrophages such as splenic red pulp macrophages and Kupffer cells. Mouse monocytes do not express CD163, and its expression differs from human CD163 in cytokine responsiveness.

This antibody detects CD163 on fixed and permeabilized cells, enabling intracellular staining. Aggressive tissue dissociation may reduce surface CD163; intracellular detection is recommended in such cases.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse splenocytes, ≤0.25 µg/test (100 µL final volume). Cell number range: 10⁵–10⁸ cells/test. Optimize concentration empirically for best performance.

Fluorochrome Information:
Super Bright 600 is a tandem dye excited at 405 nm and emits at 600 nm. Recommended filter: 610/20 bandpass. Check instrument compatibility.

When using multiple Super Bright dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific interactions.

Handling Notes:

• Light sensitive; protect vial and samples from light.
• Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-57) up to 3 days at 4°C in the dark.
• Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

CD163 (M130 antigen, Ber-Mac3, Ki-M8, SM4) is a 130 kDa membrane glycoprotein, member of the scavenger receptor cysteine-rich superfamily, and receptor for the hemoglobin-haptoglobin complex. It protects tissues from oxidative damage and mediates iron recycling through endocytosis.

CD163 is expressed on human monocytes and macrophages, upregulated by IL-10, IL-6, and dexamethasone, and shed upon LPS or PMA stimulation. It binds hemoglobin/haptoglobin complexes in a calcium- and pH-dependent manner and triggers intracellular signaling involving calcium mobilization and cytokine secretion.

For Research Use Only. Not for use in diagnostic procedures.

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