Thermo Fisher Scientific CD100 (Semaphorin-4D) Monoclonal Antibody (133-1C6)
CD100 (Semaphorin-4D) 단백질을 인식하는 Mouse IgM 단일클론 항체. 인간 및 비인간 영장류 반응성. ICC/IF 및 유세포분석(Flow Cytometry)에 적합. 단백질 A/G 정제, PBS 버퍼 내 0.05% BSA 및 sodium azide 포함. 4°C 보관, 동결 금지.
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Applications and Tested Dilution
Immunocytochemistry (ICC/IF)
- Tested Dilution: 1–2 µg/mL
Flow Cytometry (Flow)
- Tested Dilution: 1–2 µg/10⁶ cells
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Non-human primate |
| Host / Isotype | Mouse / IgM, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 133-1C6 |
| Immunogen | PHA stimulated human peripheral blood lymphocytes |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage conditions | 4°C, do not freeze |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Does not react with Horse
Target Information
Semaphorins are neuronal chemorepellants that direct pioneering neurons during nervous system development.
CD100 (also known as Semaphorin-4D), a 150 kDa homodimer, is a novel semaphorin that induces B cells to aggregate and improves their viability in vitro.
CD100 modifies CD40-CD40L B cell signaling by augmenting B cell aggregation and survival and downregulating CD23 expression.
CD72 is a lymphocyte receptor for the class IV semaphorin CD100, which is involved in regulating B cell signaling.
CD100 is expressed on resting and PHA-stimulated T cells. The protein is weakly expressed on NK cells, EBV-transformed B cells, monocytes, and tumor/peripheral blood T cell lines, and at higher density on activated T cells and B cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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