Thermo Fisher Scientific CD4 Monoclonal Antibody (RM4-5), eBioscience

Thermo Fisher Scientific CD4 Monoclonal Antibody (RM4-5), eBioscience

Applications and Tested Dilution

Product Specifications

Available Conjugated Formats

• Alexa Fluor 532, 561, 647, 660, 700
• APC, APC-eFluor 780, Biotin
• Brilliant UV series (395–805)
• Brilliant Violet series (421–786)
• eFluor series (450–615)
• FITC, PE, PE-Cyanine series, PerCP series
• NovaFluor Blue and Yellow series
• Pacific Orange, Super Bright series (436–780)
• Custom conjugation available upon request

Product Specific Information

The RM4-5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by most thymocytes, subsets of mature T cells, and dendritic cells. CD4 binds to MHC class II on antigen-presenting cells and is critical for T cell development and function. In T cells, CD4 associates with p56lck via its cytoplasmic tail. RM4-5 binding is blocked by GK1.5.

• Applications Reported: Flow cytometric analysis, immunohistochemical staining, isolation and depletion of CD4+ cells, blocking of ligand binding.
  (Functional Grade purified RM4-5, Product #16-0042 recommended for functional assays.)

• Applications Tested: Flow cytometric analysis of mouse thymocytes and splenocytes at ≤0.5 µg/test (100 µL final volume). Optimal titration recommended.

• Purity: >90% (SDS-PAGE)

• Aggregation: <10% (HPLC)

• Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD4 antigen recognizes MHC class II molecules and serves as a co-receptor for HIV. It is primarily expressed on T helper cells, but also on monocytes, macrophages, and dendritic cells. Tissue expression includes thymus, lymph nodes, tonsils, spleen, and certain brain and gut regions. CD4 aids in early T-cell activation via association with Lck and may contribute to neuronal damage in immune-mediated CNS diseases. Multiple alternatively spliced transcripts have been identified (RefSeq, July 2017).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.