Thermo Fisher Scientific PGR Monoclonal Antibody (OTI1B3), TrueMAB

Applications

• Western Blot (WB)

Tested Dilution

• 1:2,000

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using carrier-free antibody, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

The progesterone receptor (PR) is a member of the steroid family of nuclear receptors and mediates the physiological effects of progesterone, crucial in reproductive processes related to pregnancy establishment and maintenance.
PR exists as a 94 kDa (Form A) or 120 kDa (Form B) protein due to alternative translation initiation sites. PR-B is transcriptionally active, responsible for gene activation related to endometrial maintenance and inhibition of ovulation, while PR-A lacks 165 amino acids at the N-terminus, exposing an inhibitory domain that represses steroid hormone transcriptional activity.
Inactive PgR forms a multiprotein complex with heat shock proteins and immunophilins. Upon progesterone binding, the receptor undergoes conformational change, dimerizes, and binds to progesterone response elements (PRE), activating transcription.
A null mutation in the PGR gene causes pleiotropic reproductive abnormalities.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.