Thermo Fisher Scientific CCL28 Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
SEAILPIASS CCTEVSHHIS RRLLERVNMC RIQRADGDCD LAAVILHVKR RRICVSPHNH TVKQWMKVQA AKKNGKGNVC HRKKHHGKRN SNRAHQGKHE TYGHKTPY.

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human MEC (CCL28). Anti-Human MEC (CCL28)-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect hMEC by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human MEC (500-P297) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant hMEC.

Western Blot:
To detect hMEC by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. When used with compatible secondary reagents, the detection limit for Recombinant hMEC is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Package Information:
500-P297BT-1MG will be provided as 2 × 500 µg.

Target Information

MEC is a secreted CC chemokine expressed primarily by epithelial cells of the bronchioles, salivary gland, mammary gland, and colon. MEC signals through the CCR10 receptor and chemoattracts resting CD4, CD8 T-cells, and eosinophils. MEC contains six cysteines, including the four highly conserved cysteine residues present in CC chemokines.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.