Thermo Fisher Scientific TRAIL-R2 (DR5) (soluble) Polyclonal Antibody, Biotin, PeproTech

Applications

Western Blot (WB)

• Tested Dilution: 0.1–0.2 µg/mL

ELISA

• Tested Dilution: 0.25–1.0 µg/mL

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
MESALITQQD LAPQQRVAPQ QKRSSPSEGL CPPGHHISED GRDCISCKYG QDYSTHWNDL LFCLRCTRCD SGEVELSPCT TTRNTVCQCE EGTFREEDSP EMCRKCRTGC PRGMVKVGDC TPWSDIECVH KES.

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human sTRAIL Receptor-2. Anti-Human sTRAIL Receptor-2-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect hsTRAIL Receptor-2 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human sTRAIL Receptor-2 (500-P299) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant hsTRAIL Receptor-2.

Western Blot:
To detect hsTRAIL Receptor-2 by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used with compatible secondary reagents, the detection limit for Recombinant hsTRAIL Receptor-2 is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Package:
500-P299BT-1MG will be provided as 2 × 500 µg.

Target Information

DR5 (Apo2, TRAIL-R2, TRICK2, KILLER) is a death domain-containing receptor for TRAIL, mediating TRAIL-induced apoptosis. It belongs to the TNF receptor superfamily and contains an intracellular death domain. DR5 activation by TNFSF10/TRAIL/APO-2L transduces apoptosis signals. FADD, a death domain adaptor protein, is essential for DR5-mediated apoptosis. Two transcript variants encoding different isoforms and one non-coding transcript exist. Overexpression induces apoptosis and activates NF-κB. DR5 is expressed in various cell types, especially lymphocytes and spleen. Dysregulation is associated with squamous cell carcinoma and diffuse infiltrative lymphocytosis syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.