Thermo Fisher Scientific Caspase 9 (Cleaved Asp353) Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

This antibody detects endogenous levels of the fragment of activated Caspase 9 resulting from cleavage adjacent to Asp353.

Target Information

Caspase 9 (ICE-like apoptotic protease 6, ICE LAP6, apoptotic protease Mch6, apoptotic protease activating factor 3, Apaf3) is a member of the peptidase family C14 containing a CARD domain. Caspases are cysteine proteases expressed as inactive precursors that mediate apoptosis by proteolysis of specific substrates. Caspase 9 is active as a heterotetramer, located in the cytosol, and upon activation translocates to mitochondria. It participates in the caspase activation cascade responsible for apoptosis execution, cleaving and activating Caspase 3 and Caspase 6.

Binding of Caspase 9 to Apaf-1 leads to activation of the protease, which then cleaves and activates Caspase 3. Caspase 9 promotes DNA damage-induced apoptosis in an ABL1/c-Abl-dependent manner and cleaves poly(ADP-ribose) polymerase (PARP). It is expressed in various human tissues. In rats, an isoform has been identified with an alternative C-terminal peptide sequence. Mutations in Caspase 9 affect brain and neuron development. In humans, dysfunctional Caspase 9 expression varies by tissue and may be associated with cancer and neurodegenerative diseases such as Alzheimer’s Disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.