Thermo Fisher Scientific PARP1 (cleaved Asp214) Recombinant Rabbit Monoclonal Antibody (ARC0091)
PARP1 (cleaved Asp214) 단백질을 인식하는 Thermo Fisher Scientific의 재조합 토끼 단클론 항체입니다. Western blot, ELISA, IP 등에 사용 가능하며 인간과 마우스 반응성. 고순도 Affinity Chromatography 정제, 액상 형태로 안정적 저장 가능.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:2,000
ELISA
- Tested Dilution: 1 µg/mL
Immunoprecipitation (IP)
- Tested Dilution: 0.5 µg–4 µg antibody for 200 µg–400 µg extracts
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host/Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | ARC0091 |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 150–214 of human Cleaved PARP1 p25 (P09874) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol, 0.05% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2849224 |
Product Specific Information
Immunogen sequence:
QLGMIDRWYH PGCFVKNREE LGFRPEYSAS QLKGFSLLAT EDKEALKKQL PGVKSEGKRK GDEVD
Target Information
Poly ADP-Ribose Polymerase (PARP) uses nicotinamide adenine dinucleotide (oxidized form) NAD as a substrate to catalyze the transfer of ADP-ribose to various nuclear protein acceptors.
Proteolysis of PARP to its stable 85 kDa fragment is an early marker of programmed cell death (apoptosis) and is mediated by the caspase CPP32 protein.
Cleavage occurs between Asp216 and Gly217, a conserved site in PARP across species.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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