Thermo Fisher Scientific PARP1 (cleaved Asp214) Recombinant Rabbit Monoclonal Antibody (ARC0091)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

ELISA (ELISA)

1 µg/mL

-

Immunoprecipitation (IP)

0.5 µg-4 µg antibody for 200 µg-400 µg extracts of

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

ARC0091

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 150-214 of human Cleaved PARP1 p25 (P09874) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PARP1 (cleaved Asp214),uniProtId:P09874-1,ncbiNodeId:9606,antigenRange:150-214,antigenLength:1014,antigenImageFileName:MA5-35322_PARP1_cleaved_Asp214_P09874-1_Rabbit.svg,antigenImageFileNamePDP:MA5-35322_PARP1_cleaved_Asp214_P09874-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol, 0.05% BSA

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2849224

Product Specific Information

Immunogen sequence: QLGMIDRWYH PGCFVKNREE LGFRPEYSAS QLKGFSLLAT EDKEALKKQL PGVKSEGKRK GDEVD

Target Information

Poly ADP-Ribose Polymerase (PARP) uses nicotinamide adenine dinucleotide (oxidized form) NAD as a substrate to catalyse the transfer of ADP-ribose to a variety of nuclear protein acceptors. Proteolysis of PARP to its stable 85kDa fragment is an early marker of programmed cell death (apoptosis) and is mediated by the caspase CPP32 protein. Cleavage occurs between Adp216 and Gly217, a site in PARP conserved across species.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.