Thermo Fisher Scientific EDG3 Polyclonal Antibody

Applications and Tested Dilution

• Application: Immunohistochemistry (Paraffin) [IHC (P)]
• Tested Dilution: 5 µg/mL

Product Specifications

Product Specific Information

• Percent identity with other species by BLAST analysis:
  • Human, Gorilla (100%)
  • Gibbon, Monkey, Marmoset (95%)
• For IHC(P), use proteinase K antigen retrieval.
• After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.

Target Information

EDG-3 belongs to a family of G-protein coupled receptors whose ligands are lysophospholipids. The ligand for EDG-3 is sphingosine-1-phosphate.
There are eight known members of the EDG receptor family, which are implicated in mediating growth-related effects such as induction of cellular proliferation, alterations in differentiation and survival, and suppression of apoptosis.
They also evoke cellular effector functions dependent on cytoskeletal responses such as contraction, secretion, adhesion, and chemotaxis.
EDG receptors are developmentally regulated and differ in tissue distribution. They couple to multiple types of G proteins to signal through ras and MAP kinase, rho, phospholipase C, and several protein tyrosine kinases.
EDG-3 is expressed in cardiovascular, leukocyte-containing, and other tissues.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.