Thermo Fisher Scientific Ki-67 Monoclonal Antibody (5F86)
Ki-67 단백질을 표적하는 마우스 단클론 항체로, 세포 증식 마커 연구에 적합. WB, IHC, ICC/IF 등 다양한 응용 가능. 고순도 Protein A/G 정제, PBS 및 50% 글리세롤 저장. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 |
| Immunohistochemistry (IHC) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:2,000–1:5,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 5F86 |
| Immunogen | Recombinant construct containing amino acids 956–1322 of the mouse sequence expressed in and purified from E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS with 50% glycerol |
| Contains | 5 mM sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2942428 |
Target Information
Ki-67 is a nuclear protein expressed during various stages of the cell cycle, particularly during late G1, S, G2, and M phases. It contains a forkhead-associated (FHA) domain that mediates association with euchromatin at the perichromosomal layer, centromeric heterochromatin, and nucleolus. Ki-67 shows cell cycle-dependent localization: perinucleolar in G1, nuclear matrix in G2, and chromosomal in M phase. As a proliferation marker, Ki-67 is absent in G0 cells and increases from G1 through mitosis. Ki-67 antibodies are valuable for assessing proliferative activity in neoplasms and correlate with tumor grade. It is also routinely used as a neuronal marker of cell cycling and proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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