Thermo Fisher Scientific Ebola Virus NP (subtype Sudan, Zaire) Monoclonal Antibody (2603)

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

ELISA (ELISA)

1:20-1:200

-

Product Specifications

Species Reactivity

Virus

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

2603

Immunogen

Proprietary sequence

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

100 µg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.2

Contains

0.1% sodium azide

Storage conditions

4° C

Shipping conditions

Wet ice

RRID

AB_2942478

Product Specific Information

Specificity: Reactive with the NP of Sudan & Zaire sequences. Reactive with VLPs.

Purified preparations consist of >90% pure mouse monoclonal antibody which has been purified from ascites fluid by protein A chromatography. The final preparation is formulated to a protein concentration of 100 µg/mL in 0.01 M phosphate buffered saline, pH 7.2 and contains 0.1% sodium azide. Each vial contains 1.0 ml. This product contains no stabilizing proteins and should be stored at 4C until ready for use. Working dilution must be determined by the user. Suggested starting ranges are 1:10-1:50 for ICC/IF and 1:20-1:200. for ELISA.

Target Information

Ebola virus is an enveloped, negative-stranded RNA virus. This virus belongs to the family Filoviridae and has a distinctive morphology similar only to that of Marburg virus, but apparently has no antigenic characteristics of Marburg virus. Its genome is a nonsegmented RNA strand. SDS-PAGE profiles demonstrate the presence of seven structural proteins. These are the major nucleoprotein NP (104 K), glycoprotein GP (125K), L protein (180 K), and four proteins: VP40 (40 K), ViPX (35 K), VP30 (30 K) and VP24 (24 K). All the proteins are encoded by monocistronic mRNA transcripts complementary to virion RNA. GP is the major protein of the surface spikes and only this 125K viral protein is glycosylated.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.