Thermo Fisher Scientific gamma-ENaC Polyclonal Antibody
Thermo Fisher Scientific의 gamma-ENaC Polyclonal Antibody는 인간 SCNN1G 단백질(aa 160-342)에 특이적인 토끼 IgG 항체입니다. Western blot, IHC, ICC/IF, Flow cytometry 등 다양한 응용에 적합하며, 높은 특이성과 안정성을 제공합니다. 연구용으로만 사용됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:400 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:50–1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein within Human SCNN1G aa 160–342 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol, 0.2% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, avoid freeze/thaw cycles, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2854718 |
Product Specific Information
Positive Control: A431, 293T, rat kidney tissue, mouse brain tissue.
Target Information
Epithelial sodium channels (ENaC) are amiloride-sensitive members of the degenerin/epithelial sodium channel (Deg/ENaC) superfamily of ion channels. These channels share structural similarity, possessing two short intracellular termini, two transmembrane segments, and a large extracellular loop with a conserved cysteine-rich region.
There are three homologous isoforms of ENaC: alpha, beta, and gamma. ENaC plays an essential role in sodium and fluid balance across epithelial tissues such as kidney, lung, and colon. It also mediates aldosterone-dependent sodium reabsorption in the distal nephron, influencing blood pressure regulation.
ENaC is regulated partly through association with the CFTR chloride channel. Gain-of-function mutations in beta or gamma ENaC can cause Liddle’s syndrome (severe hypertension), while loss-of-function mutations in alpha or beta ENaC lead to pseudohypoaldosteronism type 1 (PHA-1).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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